Extraction of degradable biopolymer material from shrimp shell
Abstract
Chitosan is a chitin-derived natural amino-polysaccharide. Because of its natural origin and outstanding features such as biocompatibility, biodegradability, non-toxicity, and metal ion chelation, chitosan has been widely employed in a range of applications. The purpose of this research was to examine the effect of temperature on the yield percentage of chitosan. The first method went deproteination at 90°C, demineralization at 25°C, and deacetylation at 90°C. The second method ran the deproteination, demineralization and deacetylation at 25°C. The synthesized chitosan was heated in 1% acetic acid to form chitosan film. The functional groups were identified by Fourier Transform Infrared Spectrum (FT-IR). Furthermore, pH test, solubility, yield percentage and degree of deacetylation were performed. The FT-IR analysis revealed that the chitosan film had functional groups of –NH2, –CH2, –CH3, –C=O and –CN. The pH of synthesized chitosan was 8. The analysis showed that the first method had 11.9% of yield, 100% of solubility and 89% degree of deacetylation. The second method exhibited 10.2% of yield, 99% of solubility and 79% degree of deacetylation. The first method with a higher temperature of deproteination and deacetylation showed better results in synthesizing the chitosan film.